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  • 产品名称：anti-CD34antibody(CD34Molecule)(PE)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD34antibody(CD34Molecule)(PE)
详情介绍：
Clone MEC14-7
Isotype IgG2a
Specificity This antibody recognises the Murine CD34 cell surface antigen which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognises a Neuraminidase sensitive epitope. As in the Human system, CD34 antibodies in the Mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Affinity Chromatography on Protein G.
Alternative Name CD34 (CD34 Antibody Abstract)
Background The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
Gene ID 12490
NCBI Accession NP_598415
UniProt Q64314
Research Area Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Use 10 μL of Neat-1/5 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.25 mL (SM1603RT) or 1.0 mL (SM1603R) distilled water.
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative, 5 % Sucrose and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
Background publications Chabot, Jabrane-Ferrat, Bigot, Tabiasco, Provost, Golzio, Noman, Giustiniani, Bellard, Brayer, Aguerre-Girr, Meggetto, Giuriato, Malecaze, Galiacy, Jaïs, Chose, Kadouche, Chouaib, Teissié, Abitbol, Bensussan, Le Bouteiller: "A novel antiangiogenic and vascular normalization therapy targeted against human CD160 receptor." in: The Journal of experimental medicine, Vol. 208, Issue 5, pp. 973-86, 2011 (PubMed).

Chen, Zeng, Wang, Briggs, ONeill, Li, Leek, Kerr, Harris, Cai: "Roles of tetrahydrobiopterin in promoting tumor angiogenesis." in: The American journal of pathology, Vol. 177, Issue 5, pp. 2671-80, 2010 (PubMed).

Morison, Zhang, Kaituu-Lino, Fraser, Salamonsen: "The long-term actions of etonogestrel and levonorgestrel on decidualized and non-decidualized endometrium in a mouse model mimic some effects of progestogen-only contraceptives in women." in: Reproduction (Cambridge, England), Vol. 133, Issue 1, pp. 309-21, 2007 (PubMed).

Winding, NicAmhlaoibh, Misander, Høegh-Andersen, Andersen, Holst-Hansen, Heegaard, Foged, Brünner, Delaissé: "Synthetic matrix metalloproteinase inhibitors inhibit growth of established breast cancer osteolytic lesions and prolong survival in mice." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 8, Issue 6, pp. 1932-9, 2002 (PubMed).

Garlanda, Berthier, Garin, Stoppacciaro, Ruco, Vittet, Gulino, Matteucci, Mantovani, Vecchi, Dejana: "Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors." in: European journal of cell biology, Vol. 73, Issue 4, pp. 368-77, 1997 (PubMed).