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  • 产品名称：anti-CD3antibody(CD3)(FITC)

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简单介绍：
anti-CD3antibody(CD3)(FITC)
详情介绍：
Clone C363-29B (YCD3-1)
Isotype IgG2b
Specificity This antibody is specific to CD3e (Mr. 25 kDa).
Characteristics Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4
Alternative Name CD3 (CD3 Antibody Abstract)
Background CD3e, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. (Ref.1,2) CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. (Ref.1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naive T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. (Ref.1,3,4) The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody. (Ref.1)Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 12501
UniProt P22646
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Identification and enumeration of CD3+ (< / = 3 μg/10^6 cells). (Ref.1,2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-CD3 antibody (CD3) (FITC) (ABIN356218) anti-CD3 (CD3) antibody (FITC)
Background publications Luqman, Greenbaum, Lu, Bottomly: "Differential effect of interleukin 1 on naive and memory CD4+ T cells." in: European journal of immunology, Vol. 22, Issue 1, pp. 95-100, 1992 (PubMed).

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991 (PubMed).

Dianzani, Luqman, Rojo, Yagi, Baron, Woods, Janeway, Bottomly: "Molecular associations on the T cell surface correlate with immunological memory." in: European journal of immunology, Vol. 20, Issue 10, pp. 2249-57, 1990 (PubMed).

Ernst, Hobbs, Torbett, Glasebrook, Rehse, Bottomly, Hayakawa, Hardy, Weigle: "Differences in the expression profiles of CD45RB, Pgp-1, and 3G11 membrane antigens and in the patterns of lymphokine secretion by splenic CD4+ T cells from young and aged mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 5, pp. 1295-302, 1990 (PubMed).