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  • 产品名称:anti-AcrosomalVesicleProtein1(ACRV1)antibody(FITC)

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  • 产品厂商:Acris-antibodies
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简单介绍:
anti-AcrosomalVesicleProtein1(ACRV1)antibody(FITC)
详情介绍:
Immunogen Freshly ejaculated Human sperms were washed in PBS and extracted in 3% Acetic Acid,10% Glycerol, 30mM benzaminidine. The acid extract was dialyzed against 0. 2% AceticAcid and subsequently used for immunization.
Clone Hs-14
Isotype IgM
Specificity The antibody Hs-14 reacts with a 220 kDa testis-specific human intra-acrosomal protein associated with the membranes of the acrosomal vesicle.
Characteristics Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
Alternative Name ACRV1 (ACRV1 Antibody Abstract)
Background One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2
Gene ID 56
UniProt P26436
Application Notes Suitable for Flow Cytometry analysis of sperm cells using 20 μL reagent / 100 μL cellsuspension. The content of a vial (1 ml) is sufficient for 50 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer Tris buffered saline (TBS) containing 15 mM Sodium Azide as preservative and 0.2 % (w/v) high-grade BSA (protease free) as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody in the dark at 2-8 °C. DO NOT FREEZE! Avoid prolonged exposure to light.
Shelf life: One year from despatch.
Expiry Date 12 months
Supplier Images
Immunofluorescence (IF) image for anti-Acrosomal Vesicle Protein 1 (ACRV1) antibody (FITC) (ABIN238419) anti-Acrosomal Vesicle Protein 1 (ACRV1) antibody (FITC)
Background publications Peknicova, Chladek, Hozak: "Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, 2005 (PubMed).

Pavlásek, Peknicová, Ulcová-Gallová, Nováková, Reischig, Micanová, Rokyta: "[Significance of determination of intra-acrosomal proteins and sperm antibodies in human reproduction]." in: Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, Vol. 69, Issue 4, pp. 306-11, 2004 (PubMed).

Peknicová, Kyselová, Buckiová, Boubelík: "Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 47, Issue 5, pp. 311-8, 2002 (PubMed).

Chládek, P?knicová, Capková, Geussová, Teplá, Madar: "[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization]." in: Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, Vol. 65, Issue 1, pp. 28-32, 2000 (PubMed).