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  • 产品名称：anti-EMR1antibody(Egf-LikeModuleContaining,Mucin-Like,HormoneReceptor-Like1)(Bi

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-EMR1antibody(Egf-LikeModuleContaining,Mucin-Like,HormoneReceptor-Like1)(Biotin)
详情介绍：
Immunogen Cultured Macrophages. Remarks: The antigen/epitope is a 125 kDa extracellular membrane protein sensitive to 2-Mercaptoetanol.
Clone BM8
Isotype IgG2a
Specificity This antibody is useful for the detection of major subpopulations of resident tissue macrophages. It is also suitable for the detection of blood monocytes and other monocytic cells isolated from different sources by FACS. This clone is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to b-cell destruction and diabetes in a mouse diabetes model. Antigen Distribution Isolated Cells: expressed in vitro on over 80 % of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes. Tissue Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.
Cross-Reactivity (Details) Species reactivity (tested):Mouse.Also stains Human Heart Macrophages.
Purification Affinity Chromatography
Alternative Name Macrophage F4/80 Antigen (EMR1 Antibody Abstract)
Background F4/80 protein binds to Macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. F4/80 is not expressed by lymphocytes or polymorphonuclear cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480
Gene ID 13733
NCBI Accession NP_034260
UniProt Q61549
Application Notes Immunohistochemistry on Frozen Sections: 1.0 μg/mL (1/200). Immunohistochemistry on Paraffin Sections: not tested, but work well with the purifiedantibody using Proteinase K pretreatment. Recommended Positive Control: Mouse spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.5 mL distilled water
Concentration 0.2 mg/mL
Buffer PBS, pH 7.2, None, 5 mg/mL BSA
Preservative Without preservative
Precaution of Use Do Not Use Sodium Azide as Preservative.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Product cited in: Stangl, Gehrmann, Riegger, Kuhs, Riederer, Sievert, Hube, Mocikat, Dressel, Kremmer, Pockley, Friedrich, Vigh, Skerra, Multhoff: "Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 2, pp. 733-8, 2011 (PubMed).

Torzewski, Shaw, Han, Shortal, Lackner, Witztum, Palinski, Tsimikas: "Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 24, Issue 12, pp. 2307-12, 2004 (PubMed).

Background publications Schaller, Macfarlane, Rupec, Gordon, McKnight, Pfeffer: "Inactivation of the F4/80 glycoprotein in the mouse germ line." in: Molecular and cellular biology, Vol. 22, Issue 22, pp. 8035-43, 2002 (PubMed).

Rosmalen, Martin, Dobbs, Voerman, Drexhage, Haskins, Leenen: "Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 1, pp. 23-30, 2000 (PubMed).

Leenen, de Bruijn, Voerman, Campbell, van Ewijk: "Markers of mouse macrophage development detected by monoclonal antibodies." in: Journal of immunological methods, Vol. 174, Issue 1-2, pp. 5-19, 1994 (PubMed).

Smit, Duursma, Koudstaal, Hardonk, Bouma: "Infection of mice with lactate dehydrogenase-elevating virus destroys the subpopulation of Kupffer cells involved in receptor-mediated endocytosis of lactate dehydrogenase and other enzymes." in: Hepatology (Baltimore, Md.), Vol. 12, Issue 5, pp. 1192-9, 1990 (PubMed).

Kraal, Rep, Janse: "Macrophages in T and B cell compartments and other tissue macrophages recognized by monoclonal antibody MOMA-2. An immunohistochemical study." in: Scandinavian journal of immunology, Vol. 26, Issue 6, pp. 653-61, 1988 (PubMed).

Malorny, Michels, Sorg: "A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes." in: Cell and tissue research, Vol. 243, Issue 2, pp. 421-8, 1986 (PubMed).