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  • 产品名称：anti-MHCClassIH-2Dbantibody(FITC)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-MHCClassIH-2Dbantibody(FITC)
详情介绍：
Immunogen C3H. SW mouse splenocytes. (Ref. 1,2)
Clone 28-14-8
Isotype IgG2a
Specificity This antibody is specific to an epitope in the alpha 3 domain of H-2Db. It binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. (Ref.7,8) It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, (Ref.9-11) but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. (Ref.12). Customer feedback: Clone 28-14-8 does not cross-react with human cells.
Characteristics Synonyms: H-2D(B), H2-D1, H-2 class I histocompatibility antigen, D-B alpha chain
Alternative Name MHC Class I H-2 Db
Background The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5)Synonyms: D-B alpha chain, H-2 class I histocompatibility antigen, H-2D(B), H2-D1
Gene ID 14964
UniProt P01899
Application Notes Flow Cytometry: < / = 1 μg/10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-MHC Class I H-2Db antibody (FITC) (ABIN371164) anti-MHC Class I H-2Db antibody (FITC)
Background publications Kündig, Bachmann, DiPaolo, Simard, Battegay, Lother, Gessner, Kühlcke, Ohashi, Hengartner: "Fibroblasts as efficient antigen-presenting cells in lymphoid organs." in: Science (New York, N.Y.), Vol. 268, Issue 5215, pp. 1343-7, 1995 (PubMed).

Mason, Ortaldo, Young, Kumar, Bennett, Anderson: "Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2)" in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 293-303, 1995 (PubMed).

Lawlor, Zemmour, Ennis, Parham: "Evolution of class-I MHC genes and proteins: from natural selection to thymic selection." in: Annual review of immunology, Vol. 8, pp. 23-63, 1990 (PubMed).

Yewdell, Bennink: "The binary logic of antigen processing and presentation to T cells." in: Cell, Vol. 62, Issue 2, pp. 203-6, 1990 (PubMed).

Zijlstra, Bix, Simister, Loring, Raulet, Jaenisch: "Beta 2-microglobulin deficient mice lack CD4-8+ cytolytic T cells." in: Nature, Vol. 344, Issue 6268, pp. 742-6, 1990 (PubMed).

Bjorkman, Saper, Samraoui, Bennett, Strominger, Wiley: "Structure of the human class I histocompatibility antigen, HLA-A2." in: Nature, Vol. 329, Issue 6139, pp. 506-12, 1987 (PubMed).

Allen, Fraser, Flyer, Calvin, Flavell: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, 1986 (PubMed).

Allen, Wraith, Pala, Askonas, Flavell: "Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites." in: Nature, Vol. 309, Issue 5965, pp. 279-81, 1984 (PubMed).

Orn, Goodenow, Hood, Brayton, Woodward, Harmon, Frelinger: "Product of a transferred H-2Ld gene acts as restriction element for LCMV-specific killer T cells." in: Nature, Vol. 297, Issue 5865, pp. 415-7, 1982 (PubMed).

Woodward, Orn, Harmon, Goodenow, Hood, Frelinger: "Specific recognition of the product of a transferred major histocompatibility complex gene by cytotoxic T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 11, pp. 3613-7, 1982 (PubMed).

Ozato, Hansen, Sachs: "Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 125, Issue 6, pp. 2473-7, 1981 (PubMed).

Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, 1981 (PubMed).