anti-alphaTubulinantibody(TUBA1)(AA400-450)(Biotin) 抗体网

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产品名称：anti-alphaTubulinantibody(TUBA1)(AA400-450)(Biotin)
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产品厂商：GenScript
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anti-alphaTubulinantibody(TUBA1)(AA400-450)(Biotin)

详情介绍：

Product Details anti-alpha Tubulin Antibody
Product Details anti-alpha Tubulin Antibody
Immunogen	KLH-coupled synthetic peptide within AA 400-450 of human alpha-tubulin .
Clone	1F4E3
Isotype	IgG
Specificity	Anti-alpha-tubulin Antibody[Biotin], mAb, Mouse recognizes Mouse, Human, Hamster, Monkey, Rabbit and Pig. The antibody has not yet been tested in other species.
Cross-Reactivity (Details)	Cross reactivity in other species has not yet been tested.
Purification	alpha-tubulin Antibody[Biotin], mAb, Mouse is the biotin conjugated alpha-tubulin Antibody, mAb, Mouse(ABIN387714) purified from mice ascites by protein A affinity column.

Target Details alpha Tubulin
Target Details alpha Tubulin
Alternative Name	alpha Tubulin (TUBA1 Antibody Abstract)
Research Area	Signaling, Cytoskeleton
Pathways	Microtubule Dynamics

Application Details
Application Details
Application Notes	Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/mL Western blot: 0.1-0.5 µg/mL Other applications: user-optimized
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Buffer	PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
Preservative	Sodium azide
Precaution of Use	WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice	Avoid repeated freezing and thawing cycles.
Storage	4 °C/-20 °C
Storage Comment	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Images
Images
Supplier Images	cAMP Competitive ELISA1.ELISA plate was coated with goat anti mouse IgG antibody 2.An... cAMP Competitive ELISA1.ELISA plate was coated with goat anti mouse IgG antibody 2.Anti-cAMP monoclonal antibody (ABIN387719) at appropriate dilution and cAMP standards or testing compounds were added into appropriate reaction wells. 3.After a period of incubation, cAMP-HRP conjugate (ABIN1536525) was added followed by proper period of incubation. 4.ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm. cAMP Competitive ELISA1.ELISA plate was coated with goat anti mouse IgG antibody 2.Anti-cAMP monoclonal antibody (ABIN387719) at appropriate dilution and cAMP standards or testing compounds were added into appropriate reaction wells. 3.After a period of incubation, cAMP-HRP conjugate (ABIN1536525) was added followed by proper period of incubation. 4.ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.

ProductDetails: References for anti-alpha Tubulin antibody (ABIN387720)
ProductDetails: References for anti-alpha Tubulin antibody (ABIN387720)
Product cited in:	Martinez-Espinosa, Yang, Gonzalez-Perez, Xia, Lingle: "Knockout of the BK ?2 subunit abolishes inactivation of BK currents in mouse adrenal chromaffin cells and results in slow-wave burst activity." in: The Journal of general physiology, Vol. 144, Issue 4, pp. 275-95, 2014 (PubMed). Capecci, Forgac: "The function of vacuolar ATPase (V-ATPase) a subunit isoforms in invasiveness of MCF10a and MCF10CA1a human breast cancer cells." in: The Journal of biological chemistry, Vol. 288, Issue 45, pp. 32731-41, 2013 (PubMed).